Active Fibroblast Growth Factor 3 (FGF3)
HBGF3; INT2; Heparin-binding growth factor 3; Murine Mammary Tumor Virus Integration Site(v-int-2)Oncogene Homolog; INT-2 Proto-Oncogene Protein; Proto-oncogene Int-2
- Product No.APC906Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer Formulation100mMNaHCO3, 500mMNaCl, pH8.3, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- TraitsFreeze-dried powder
- Purity> 90%
- Isoelectric Point11.0
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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FOB
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ACTIVITY TEST of the Active Fibroblast Growth Factor 3 (FGF3)
Fibroblast Growth Factor 3 (FGF3) also known as INT-2 proto-oncogene protein is a member of the fibroblast growth factor family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. Besides, Fibroblast Growth Factor Receptor 2 (FGFR2) has been identified as an interactor of FGF3, thus a binding ELISA assay was conducted to detect the interaction of recombinant human FGF3 and recombinant human FGFR2. Briefly, FGF3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to FGFR2-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-Fibroblast Growth Factor Receptor 2 (FGFR2) pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of FGF3 and FGFR2 was shown in Figure 1, and this effect was in a dose dependent manner.
Figure. The binding activity of FGF3 with FGFR2.
USAGE of the Active Fibroblast Growth Factor 3 (FGF3)
Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Fibroblast Growth Factor 3 (FGF3)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Fibroblast Growth Factor 3 (FGF3)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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