Active Fibroblast Growth Factor 21 (FGF21)

  • Active Fibroblast Growth Factor 21 (FGF21) Packages (Simulation)
  • Active Fibroblast Growth Factor 21 (FGF21) Packages (Simulation)
  • Active Fibroblast Growth Factor 21 (FGF21) Figure. Gene Sequencing (Extract)
  • APC918Ra01.jpg Figure. SDS-PAGE
  • Active Fibroblast Growth Factor 21 (FGF21) Figure. Western Blot
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

ACTIVITY TEST of the Active Fibroblast Growth Factor 21 (FGF21)

Figure. The binding activity of FGF21 with FGFR1.
Fibroblast growth factor 21 (FGF21) is a member of the fibroblast growth factor (FGF) family and specifically a member of the endocrine subfamily which includes FGF23 and FGF15/19. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF21 action through one of the FGF21 receptors thus requires interaction with a co-receptor, designated β-klotho. Besides, Fibroblast Growth Factor Receptor 1 (FGFR1) has been identified as an interactor of FGF21, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat FGF21 and recombinant rat FGFR1. Briefly, FGF21 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to FGFR1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-FGF21 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of FGF21 and FGFR1 was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE of the Active Fibroblast Growth Factor 21 (FGF21)

Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE of the Active Fibroblast Growth Factor 21 (FGF21)

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY of the Active Fibroblast Growth Factor 21 (FGF21)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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