Active Fibroblast Growth Factor 20 (FGF20)

  • Active Fibroblast Growth Factor 20 (FGF20) Packages (Simulation)
  • Active Fibroblast Growth Factor 20 (FGF20) Packages (Simulation)
  • APC912Hu01.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

ACTIVITY TEST of the Active Fibroblast Growth Factor 20 (FGF20)

Fibroblast growth factor 20 (FGF20) is a member of the fibroblast growth factor family. The fibroblast growth factors possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. It is identified that Fibroblast Growth Factor 20 (FGF20)-FGF receptor 1 (FGFR1) signaling is essential for cochlear hair cell (HC) and supporting cell (SC) differentiation. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human FGF20 and recombinant human FGFR1. Briefly, FGF20 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to FGFR1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-FGF20 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human FGF20 and recombinant human FGFR1 was shown in Figure 1, the EC50 for this effect is 0.17 ug/mL.

USAGE of the Active Fibroblast Growth Factor 20 (FGF20)

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE of the Active Fibroblast Growth Factor 20 (FGF20)

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY of the Active Fibroblast Growth Factor 20 (FGF20)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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