Active Fibroblast Growth Factor 18 (FGF18)

ZFGF5

  • Active Fibroblast Growth Factor 18 (FGF18) Packages (Simulation)
  • Active Fibroblast Growth Factor 18 (FGF18) Packages (Simulation)
  • Active Fibroblast Growth Factor 18 (FGF18) Figure . Gene Sequencing (extract)
  • APC907Hu01.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

ACTIVITY TEST of the Active Fibroblast Growth Factor 18 (FGF18)

Fibroblast Growth Factor-18 (FGF18) is a trophic factor for mature chondrocytes
and their progenitors. It has been reported to have significant anabolic effects on
cartilage. FGF18 plays a central role in skeletal growth and
development.Besides,Fibroblast Growth Factor Receptor 3 (FGFR3) has been
identified as an interactor of FGF18, thus a binding ELISA assay was conducted
to detect the interaction of recombinant human FGF18 and recombinant human
FGFR3. Briefly, FGF18 were diluted serially in PBS, with 0.01% BSA (pH 7.4).
Duplicate samples of 100μl were then transferred to FGFR3-coated microtiter
wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated
for 1h with anti-FGF18 pAb, then aspirated and washed 3 times. After incubation
with HRP labelled secondary antibody, wells were aspirated and washed 3 times.
With the addition of substrate solution, wells were incubated 15-25 minutes at
37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately.
The binding activity of FGF18 and FGFR3 was shown in Figure 1, and this effect
was in a dose dependent manner.

USAGE of the Active Fibroblast Growth Factor 18 (FGF18)

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE of the Active Fibroblast Growth Factor 18 (FGF18)

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY of the Active Fibroblast Growth Factor 18 (FGF18)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Catalog No. Organism species: Homo sapiens (Human) Applications (RESEARCH USE ONLY!)
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