Active Amiloride Binding Protein 1 (ABP1)

AOC1; DAO; KAO; Histaminase; Kidney amine oxidase; Diamine Oxidase; Amiloride-sensitive amine oxidase; Amine oxidase copper domain-containing protein 1

  • Active Amiloride Binding Protein 1 (ABP1) Packages (Simulation)
  • Active Amiloride Binding Protein 1 (ABP1) Packages (Simulation)
  • Active Amiloride Binding Protein 1 (ABP1) Figure. Gene Sequencing (Extract)
  • APA656Hu61.png Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

ACTIVITY TEST of the Active Amiloride Binding Protein 1 (ABP1)

Figure. The binding activity of DAO with DDC.
Diamine oxidase (DAO), also known as histaminase, is an enzyme involved in the metabolism, oxidation, and inactivation of histamine in animals. Highest content is observed in the digestive tract and placenta. It is also secreted by eosinophils. In case of a shortage of diamine oxidase in the human body, it may appear as an allergy or histamine intolerance. Besides, Dopa Decarboxylase (DDC) has been identified as an interactor of DAO, thus a binding ELISA assay was conducted to detect the interaction of recombinant human DAO and recombinant human DDC. Briefly, DAO were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to DDC-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-DAO pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of DAO and DDC was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE of the Active Amiloride Binding Protein 1 (ABP1)

Reconstitute in 10mM PBS (pH7.6) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE of the Active Amiloride Binding Protein 1 (ABP1)

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY of the Active Amiloride Binding Protein 1 (ABP1)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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