Active Cytochrome P450 1A2 (CYP1A2)

CP12; P3-450; P450(PA); Cytochrome P450,Family 1,Subfamily A,Polypeptide 2

  • Active Cytochrome P450 1A2 (CYP1A2) Packages (Simulation)
  • Active Cytochrome P450 1A2 (CYP1A2) Packages (Simulation)
  • Active Cytochrome P450 1A2 (CYP1A2) Gene sequencing
  • APD294Hu01.jpg SDS-PAGE
  • Active Cytochrome P450 1A2 (CYP1A2) Figure. Western Blot; Sample: Recombinant CYP1A2, Human.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

ACTIVITY TEST of the Active Cytochrome P450 1A2 (CYP1A2)

CYP1A2 (Cytochrome P450 1A2) belongs to the group of proteins which contains heme as a cofactor. CYP1A2 oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Besides, ASAH1 (Acid ceramidase) has been identified as an interactor of CYP1A2 through affinity capture-MS. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human CYP1A2 and recombinant human ASAH1. Briefly, CYP1A2 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to ASAH1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-CYP1A2 mAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of CYP1A2 and ASAH1 was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE of the Active Cytochrome P450 1A2 (CYP1A2)

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE of the Active Cytochrome P450 1A2 (CYP1A2)

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY of the Active Cytochrome P450 1A2 (CYP1A2)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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