Active Cytochrome P450 1A1 (CYP1A1)
CYP1; AHH; AHRR; CP11; CYP1; P1-450; P450-C; P450DX; Cytochrome P450,Subfamily I(Aromatic Compound-Inducible),Polypeptide 1
- Product No.APD295Ra01
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- TraitsFreeze-dried powder
- Purity> 95%
- Isoelectric Point6.8
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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- Packages (Simulation)
- Packages (Simulation)
- Figure. Western Blot; Sample: Recombinant CYP1A1, Rat.
- ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Cytochrome P450 1A1 (CYP1A1)
Cytochrome P450 1A1 (CYP1A1) is a member of Cytochromes P450 superfamily of enzymes. Cytochromes P450 are a group of heme-thiolate monooxygenases. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. CYP1A1 is also known as AHH (aryl hydrocarbon hydroxylase). It is involved in the metabolic activation of aromatic hydrocarbons (polycyclic aromatic hydrocarbons, PAH). Besides, Heat Shock 70kDa Protein 4 (HSPA4) has been identified as an interactor of CYP1A1, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat CYP1A1 and recombinant rat HSPA4. Briefly, CYP1A1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to HSPA4-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-CYP1A1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of CYP1A1 and HSPA4 was shown in Figure 1, and this effect was in a dose dependent manner.
USAGE of the Active Cytochrome P450 1A1 (CYP1A1)
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Cytochrome P450 1A1 (CYP1A1)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Cytochrome P450 1A1 (CYP1A1)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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|Catalog No.||Organism species: Rattus norvegicus (Rat)||Applications (RESEARCH USE ONLY!)|
|RPD295Ra01||Recombinant Cytochrome P450 1A1 (CYP1A1)||Positive Control; Immunogen; SDS-PAGE; WB.|
|APD295Ra01||Active Cytochrome P450 1A1 (CYP1A1)||Cell culture; Activity Assays.|
|PAD295Ra01||Polyclonal Antibody to Cytochrome P450 1A1 (CYP1A1)||WB; IHC; ICC; IP.|
|MAD295Ra21||Monoclonal Antibody to Cytochrome P450 1A1 (CYP1A1)||WB; IHC; ICC; IP.|
|SED295Ra||ELISA Kit for Cytochrome P450 1A1 (CYP1A1)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|KSD295Ra01||ELISA Kit DIY Materials for Cytochrome P450 1A1 (CYP1A1)||Main materials for "Do It (ELISA Kit) Yourself".|