Active Complement Factor B (CFB)
CF-B; BF; BFD; CFAB; GBG; H2-Bf; PBF2; B-Factor; Properdin; Properdin factor B; C3/C5 convertase; Glycine-rich beta glycoprotein
- Product No.APC011Hu03
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- TraitsFreeze-dried powder
- Purity> 90%
- Isoelectric Point6.7
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
-
FOB
US$ 274
For more details, please contact local distributors!US$ 684
For more details, please contact local distributors! US$ 1368
For more details, please contact local distributors! US$ 4104
For more details, please contact local distributors! US$ 10260
For more details, please contact local distributors!
ACTIVITY TEST of the Active Complement Factor B (CFB)
Complement Factor B (CFB) a component of the alternative pathway of complement activation. Upon activation of the alternative pathway, it is cleaved by complement factor D yielding the noncatalytic chain Ba and the catalytic subunit Bb. The active subunit Bb is a serine protease that associates with C3b to form the alternative pathway C3 convertase. The method of functional assay of CFB was tested in hemolysis assays. Two-fold dilute the recombinant human CFB with 0.9% NaCl, 2mmol/L MgCl2 ,and then add same volume of 1% rabbit erythrocyte (RaE) in 8mmol/L EDTA, the negative control only without MgCl2. All the samples incubated at 37℃. After 3 hours later, take 10μL supernatant and 90μL TMB incubated at 37℃ for 5-10 minutes. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The results are shown in Figure 1. It was obvious that the minimal effective concentration of CFB is 0.3125μg/mL.(A) 1% rabbit erythrocyte (RaE) treated with 0.3125μg/mL CFB for 1h
(B) Negative control (1% RaE treated with 0.3125ug/mL CFB, 8mmol/L EDTA) without MgCl2.
Figure. The hemolysis effection of recombinant human CFB
Figure. The hemolysis activity of recombinant human CFB
USAGE of the Active Complement Factor B (CFB)
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Complement Factor B (CFB)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Complement Factor B (CFB)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
INCREMENT SERVICES
BCA Protein Quantification Kit
Molecular Mass Marker for Protein
Monoclonal Antibody Customized Service
Polyclonal Antibody Customized Service
Protein Activity Test Experiment Service
Electrophoretic Mobility Shift Assay (EMSA) Experiment Service
Buffer
Lentivirus Packaging Experiment Service
Adenovirus Packaging Experiment Service
Real Time PCR Experimental Service
Spike RBD Protein (S-RBD)
Protein G
Protein A
Related products
Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
RPC011Hu05 | Recombinant Complement Factor B (CFB) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPC011Hu03 | Recombinant Complement Factor B (CFB) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPC011Hu02 | Recombinant Complement Factor B (CFB) | Positive Control; Immunogen; SDS-PAGE; WB. |
EPC011Hu61 | Eukaryotic Complement Factor B (CFB) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPC011Hu04 | Recombinant Complement Factor B (CFB) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPC011Hu01 | Recombinant Complement Factor B (CFB) | Positive Control; Immunogen; SDS-PAGE; WB. |
APC011Hu03 | Active Complement Factor B (CFB) | Cell culture; Activity Assays. |
PAC011Hu03 | Polyclonal Antibody to Complement Factor B (CFB) | WB; IHC |
PAC011Hu02 | Polyclonal Antibody to Complement Factor B (CFB) | WB; IHC; ICC; IP. |
PAC011Hu01 | Polyclonal Antibody to Complement Factor B (CFB) | WB; IHC |
PAC011Hu04 | Polyclonal Antibody to Complement Factor B (CFB) | WB; IHC; ICC; IP. |
LAC011Hu71 | Biotin-Linked Polyclonal Antibody to Complement Factor B (CFB) | WB; IHC; ICC. |
MAC011Hu22 | Monoclonal Antibody to Complement Factor B (CFB) | WB; IHC; ICC; IP. |
WEC011Hu | Wide-range ELISA Kit for Complement Factor B (CFB) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SEC011Hu | ELISA Kit for Complement Factor B (CFB) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMC011Hu | Multiplex Assay Kit for Complement Factor B (CFB) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |