Active Cathepsin V (CTSV)

CTS-L2; CTSL2; CATL2; CTSU; CTS-V; Cathepsin L2

  • Active Cathepsin V (CTSV) Packages (Simulation)
  • Active Cathepsin V (CTSV) Packages (Simulation)
  • APC970Hu01.jpg SDS-PAGE
  • Active Cathepsin V (CTSV) Figure. Western Blot; Sample: Recombinant CTSV, Human.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

ACTIVITY TEST of the Active Cathepsin V (CTSV)

Cathepsin V (CTSV) is a lysosomal cysteine proteinase which belongs to peptidase C1 family. It is expressed in normal human thymus, testis and corneal epithelium. Cathepsin V plays an important role in corneal physiology and mediates degradation of invariant chain in human thymus. Besides, Retinoblastoma Protein 1 (RB1) has been identified as an interactor of CTSV, thus a binding ELISA assay was conducted to detect the interaction of recombinant human CTSV and recombinant human RB1. Briefly, CTSV were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to RB1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-CTSV pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of CTSV and RB1 was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE of the Active Cathepsin V (CTSV)

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE of the Active Cathepsin V (CTSV)

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY of the Active Cathepsin V (CTSV)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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