Active Catechol-O-Methyltransferase (COMT)
- Product No.APJ365Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- TraitsFreeze-dried powder
- Purity> 90%
- Isoelectric Point5.6
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
-
FOB
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ACTIVITY TEST of the Active Catechol-O-Methyltransferase (COMT)
Catechol-O-Methyltransferase (COMT) is one of several enzymes that degrade catecholamines (such as dopamine, epinephrine, and norepinephrine), catecholestrogens, and various drugs and substances having a catechol structure. Two isoforms of COMT are produced: the soluble short form (S-COMT) and the membrane bound long form (MB-COMT). COMT is involved in the inactivation of the catecholamine neurotransmitters (dopamine, epinephrine, and norepinephrine). The enzyme introduces a methyl group to the catecholamine, which is donated by S-adenosyl methionine (SAM). Any compound having a catechol structure, like catecholestrogens and catechol-containing flavonoids, are substrates of COMT. Besides, UDP glucuronosyltransferase 2 family, polypeptide B1 (UGT2B1) has been identified as an interactor of COMT, thus a binding ELISA assay was conducted to detect the interaction of recombinant human COMT and recombinant human UGT2B1. Briefly, COMT were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to UGT2B1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-COMT pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of COMT and UGT2B1 was shown in Figure 1, and this effect was in a dose dependent manner.
Figure. The binding activity of COMT with UGT2B1.
USAGE of the Active Catechol-O-Methyltransferase (COMT)
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Catechol-O-Methyltransferase (COMT)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Catechol-O-Methyltransferase (COMT)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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