Active Calpain 3 (CAPN3)
CANPL3; p94; LGMD2; LGMD2A; nCL1; Calcium-activated neutral proteinase 3; Muscle-specific calcium-activated neutral protease 3; New calpain 1
- Product No.APC960Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- TraitsFreeze-dried powder
- Purity> 95%
- Isoelectric Point5.5
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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- Packages (Simulation)
- Packages (Simulation)
- Figure. SDS-PAGE
- Figure. Western Blot
- ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Calpain 3 (CAPN3)
Figure. The binding activity of CAPN3 with TTN.
Calpain 3 is a calcium-dependent cysteine protease mainly expressed in skeletal muscle. In humans, calpain 3 is encoded by the CAPN3 gene. This gene encodes a muscle-specific member of the calpain large subunit family that specifically binds to titin. Mutations in this gene are associated with limb-girdle muscular dystrophies type 2A. Alternate promoters and alternative splicing result in multiple transcript variants encoding different isoforms and some variants are ubiquitously expressed. Besides, Titin (TTN) has been identified as an interactor of CAPN3, thus a binding ELISA assay was conducted to detect the interaction of recombinant human CAPN3 and recombinant human TTN. Briefly, CAPN3 were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to TTN-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-CAPN3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of CAPN3 and TTN was shown in Figure 1, and this effect was in a dose dependent manner.
USAGE of the Active Calpain 3 (CAPN3)
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Calpain 3 (CAPN3)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Calpain 3 (CAPN3)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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|Catalog No.||Organism species: Homo sapiens (Human)||Applications (RESEARCH USE ONLY!)|
|APC960Hu01||Active Calpain 3 (CAPN3)||Cell culture; Activity Assays.|
|RPC960Hu01||Recombinant Calpain 3 (CAPN3)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAC960Hu01||Polyclonal Antibody to Calpain 3 (CAPN3)||WB; IHC; ICC; IP.|
|MAC960Hu22||Monoclonal Antibody to Calpain 3 (CAPN3)||WB; IHC; ICC; IP.|
|SEC960Hu||ELISA Kit for Calpain 3 (CAPN3)||Enzyme-linked immunosorbent assay for Antigen Detection.|