Active Adenosylhomocysteinase (AHCY)
SAHH; AdoHcyase; S-Adenosylhomocysteine Hydrolase; S-adenosyl-L-homocysteine hydrolase
- Product No.APG465Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- TraitsFreeze-dried powder
- Purity> 95%
- Isoelectric Point5.9
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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- Packages (Simulation)
- Packages (Simulation)
- Figure. SDS-PAGE
- Figure. Western Blot
- ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Adenosylhomocysteinase (AHCY)
Figure. The binding activity of AHCY with MHCB.
Adenosylhomocysteinase (S-adenosylhomocysteine synthase, S-adenosylhomocysteine hydrolase, adenosylhomocysteine hydrolase, S-adenosylhomocysteinase, SAHase, AdoHcyase, AHCY) is an enzyme that converts S-adenosylhomocysteine to homocysteine and adenosine. The enzyme contains one tightly bound NAD per subunit. Besides, Major Histocompatibility Complex Class I B (MHCB) has been identified as an interactor of AHCY, thus a binding ELISA assay was conducted to detect the interaction of recombinant human AHCY and recombinant human MHCB. Briefly, AHCY were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to MHCB-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-AHCY pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of AHCY and MHCB was shown in Figure 1, and this effect was in a dose dependent manner.
USAGE of the Active Adenosylhomocysteinase (AHCY)
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Adenosylhomocysteinase (AHCY)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Adenosylhomocysteinase (AHCY)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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|Catalog No.||Organism species: Homo sapiens (Human)||Applications (RESEARCH USE ONLY!)|
|RPG465Hu01||Recombinant Adenosylhomocysteinase (AHCY)||Positive Control; Immunogen; SDS-PAGE; WB.|
|APG465Hu01||Active Adenosylhomocysteinase (AHCY)||Cell culture; Activity Assays.|
|PAG465Hu01||Polyclonal Antibody to Adenosylhomocysteinase (AHCY)||WB; IHC; ICC; IP.|
|MAG465Hu21||Monoclonal Antibody to Adenosylhomocysteinase (AHCY)||WB; IHC; ICC; IP.|
|SEG465Hu||ELISA Kit for Adenosylhomocysteinase (AHCY)||Enzyme-linked immunosorbent assay for Antigen Detection.|