TGFB2 is present at elevated levels in the aqueous humor of patients with primary open angle glaucoma (POAG). Studies have shown that TGFB2 influences cultured trabecular meshwork cells.TGFB2 reduced outflow facility when perfused into cultured human anterior segments. Furthermore, TGFB2 affected the extracellular matrix of the trabecular meshwork in a manner that was consistent with the observed reduction in outflow facility. Although the distribution of accumulated fibrillar material was different in these perfused eyes than that in POAG, the difference could have been due to variation in biomechanical environment for trabecular meshwork cells in cultured anterior segments compared with the living eye. elevated TGFB2 levels in the aqueous humor played a role in the pathogenesis of the ocular hypertension in POAG.